![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K542009:Design
Designed by: Anthony Vuong Group: iGEM11_Lethbridge (2011-09-24)
pLacI Regulated AG43
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 935
Illegal NgoMIV site found at 2159
Illegal NgoMIV site found at 2669
Illegal NgoMIV site found at 2690
Illegal AgeI site found at 1685
Illegal AgeI site found at 2429
Illegal AgeI site found at 2843
Illegal AgeI site found at 3085 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS (https://parts.igem.org/Part:BBa_K346007 BBa_K346007). A promoter and RBS was needed.
Source
BBa_J04500 and BBa_K346007 were obtained from iGEM Distribution plates.