Composite

Part:BBa_K542009:Design

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-24)


pLacI Regulated AG43


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 2159
    Illegal NgoMIV site found at 2669
    Illegal NgoMIV site found at 2690
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2429
    Illegal AgeI site found at 2843
    Illegal AgeI site found at 3085
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS (https://parts.igem.org/Part:BBa_K346007 BBa_K346007). A promoter and RBS was needed.


Source

BBa_J04500 and BBa_K346007 were obtained from iGEM Distribution plates.

References